CD4~+CD25- CD45RB~(high) T细胞转移性肠炎模型的建立

目的建立CD4+CD25-CD45RBhighT细胞转移性肠炎模型,并分析其在炎性肠病中的研究价值。方法流式细胞仪分选和纯化CD4+CD25-CD45RBhighT细胞并通过静脉注射移植到Rag1-/-小鼠,记录每组小鼠体质量变化,根据标准程序制作结肠组织切片进行HE染色检查,分离肠黏膜固有层CD4+T细胞并采用ELISA方法检测TNF-α和IFN-γ的表达水平。结果 CD4+CD25-CD45RBhighT细胞重建Rag1-/-小鼠3周后出现体质量下降,至第6周时体质量下降更加急剧并伴有明显腹泻。结肠组织切片结果发现明显的炎性细胞浸润,结肠上皮出现局灶性溃疡,炎症进一步蔓延至黏膜下层。结肠黏膜固有层CD4+T细胞TNF-α和IFN-γ的表达水平明显高于对照组。结论CD4+CD25-CD45RBhighT细胞转移性肠炎模型在研究炎性肠病的发病机制中具有重要作用。     

聚合物修饰膜中中继体扩散系数的测定

采用计时电流法测定中继体Os(bpy)23 /3 在聚合物AQ单层和AQ与PVP组成的复合膜修饰电极中的扩散系数分别为4.57×10-9cm2/s和1.44×10-8cm2/s。结果显示复合膜中中继体的扩散系数比单层膜中的扩散系数大3倍。     

EFFECTS OF FILTER MEMBRANES ADSORBING INFLAMMATORY MEDIATORS IN HEMOFILTRATION

Sepsisischaracterizedbyreleaseofexcessiveamountofproinflammatorycytokinesinthecircu lation ,leadingtoa generalizedanduncontrolledhostresponse .Aconsiderablenumberofexperimen talandclinicalinvestigatorshavereportedemploy inghemofiltration (HF)toeliminatecytokinesandchemokines .Thisexcessiveinflammatoryresponsecouldbedownregulatedwithremovalofcytokinesandothermediators .Thesedatashowedthatthemechanismofmediatorseliminationwaslinkedtoconvectionandadsorptionbyhemofiltration .Ad sorptionappearstob…     

山莨菪碱对大鼠急性坏死性胰腺炎肾损伤的保护作用

目的　探讨大鼠急性坏死性胰腺炎 (ANP)肾损伤的机制及山莨菪碱的治疗作用。方法　采用 35 g·L-1(3.5 %)牛磺胆酸钠溶液逆行注射胰胆管建立鼠的ANP模型。观察炎性介质白细胞介素IL 1β、IL 6、肿瘤坏死因子 (TNFα)、内毒素、淀粉酶、磷酯酶A2 (PLA2 )的变化和山莨菪碱的作用。结果　ANP可以诱导炎性介质的明显升高 ,并与肾损伤的严重程度正相关。而山莨菪碱可抑制ANP的炎性介质过度表达 ,减轻肾损伤的程度。结论　炎性介质参与了ANP对肾的损伤 ,山莨菪碱对ANP肾损伤有治疗作用。     

SDF-1α/CXCR4通路在大鼠弥漫性轴索损伤中的作用

目的通过探讨弥漫性轴索损伤(diffuse axonal injury,DAI)后脑组织SDF-1α及CXCR4的表达以及抑制该通路后对外周炎症细胞浸润、轴索损伤和β-APP及MMP-9表达的影响,揭示SDF-1α/CXCR4通路在DAI后炎性损伤中的作用。方法采用大鼠头颅瞬间旋转装置制备大鼠DAI模型,于造模后6、24、48、72h取脑组织,采用Western blot测定脑组织SDF-1α及其受体CXCR4的表达,并且通过给予该通路特异性抑制剂,观察外周炎症细胞浸润后相关蛋白β-APP、MMP-9的表达,及脑脊液中轴索损伤相关蛋白MBP的含量,揭示其在DAI后的炎性损伤中的作用及其机制。结果 DAI模型组皮层SDF-1α及CXCR4的表达量较对照组均显著增加,测定DAI后24h,脑皮层组织中MPO阳性细胞数、β-APP、MMP-9的表达均较对照组显著升高,脑脊液中MBP的水平也较对照组明显升高;给予CXCR4特异性抑制剂AMD3100后,脑皮层组织中MPO阳性细胞数、β-APP、MMP-9的表达较DAI模型组均明显下降,并且脑脊液中MBP水平也较DAI模型组显著下降。结论 SDF-1α/CXCR4通路活化在介导大鼠DAI后脑组织的炎性损伤中具有重要作用。     

The effect of Levocarnitine on nutritional status and lipid metabolism during long-term maintenance hemodialysis

Objective To investigate the effect of Levocarnitine on lipid metabolism and nutritional status of maintenance hemodialysis (MHD) patients and possible mechanism. Methods A total of 40 MHD patients [mean age (53.5±7.1) years] who underwent normal hemodialysis more than 6 months were randomly classified into two groups, Levocarnitine supplemented group (LS-G) (n=20; Levocarnitine supplementation after each normal hemodialysis session, at a dose of 1.0 g/day by intravenous administration) and control group (C-G) (n=20; normal hemodialysis). Before treatment, one month and three months after treatment we respectively measured or observed the following items, the tolerance to hemodialysis, carnitine level in plasma, C-reactive protein, IL-6, TNF-α, percentage of neutrophil, and some relevant nutritional parameters, such as lipid profile, transferrin, total protein, albumin and prealbumin levels. Comparative analysis was conducted between the two groups. Results In LS-G three months after treatment, the levels of carnitine, hemoglobin, and prealbumin in plasma were significantly increased (P<0.05), but C-reactive protein, neutrophil percentage, low-density lipoprotein and triglyceride were significantly decreased (P<0.05) in contrast to those in C-G and before treatment. Transferrin, total protein, and albumin were elevated in LS-G, with no statistical significance. Conclusion There was a significant improvement of lipid metabolism and nutritional status for the long-term maintenance hemodialysis patients with Levocarnitine supplementation. And this improvement is related to the decrease of inflammatory factors.     

血液净化对肾综合征出血热炎症介质的清除作用

目的　通过观察连续性肾替代治疗 (CRRT)对肾病综合征出血热 (HFRS)患者肿瘤坏死因子α(TNF α)、白介素 6(IL 6 )及内皮素 1(ET 1)的清除作用 ,探讨炎症介质在HFRS中的作用。方法　将诊断为HFRS的中型及以上类型患者 4 0例随机分为两组 :CVVH组 2 0例行CVVH ,HD组 2 0例行HD。用ELISA法检测TNF α和IL 6浓度 ,放射免疫法测定ET 1浓度。结果　①CVVH组及HD组少尿天数 (3.0± 2 .1,6 .0± 3.4 )、并发症发生率 (2 5 % ,4 0 % )及病死率 (15 % ,2 5 % )差异均有显著性 (P <0 .0 1)。②两组治疗前后血肌酐和尿素氮下降有显著性差异 (P <0 .0 1)。③CVVH组治疗后血中TNF α、IL 6和ET 1均较治疗前明显降低 ,差异均有显著性意义 (P均 <0 .0 1) ;HD组治疗前后血中TNF α、IL 6和ET 1无显著性差异 (P >0 .0 5 )。④CVVH组滤出液中可持续检测到IL 6及ET 1,未检测到TNF α。透出液中未检测到上述炎症介质。结论　连续性血液净化治疗可清除炎症介质 ,改善HFRS的病理生理过程 ,改善肾功能和预后 ,减少并发症 ,降低病死率 ,是治疗HFRS的最佳方法     

考虑环保意识和出行习惯的公交出行选择行为模型

为解决公交出行选择行为、环保意识与出行习惯之间缺少定量分析交互作用的问题，基于计划行为理论(TPB)，结合习惯驱动行为理论，构建公交出行选择行为结构方程基本模型A，增加环保意识的扩展模型B，以及涵盖环保意识和出行习惯的扩展模型C，对比分析公交出行选择行为各影响因素之间的定量关系.最后，将模型运用于重庆市实例分析，共回收有效问卷401份.结果表明：模型C的拟合效果及解释性最好，模型B其次；环保意识、出行习惯均对公交出行方式选择行为存在影响，但影响程度低于行为意向和知觉行为控制.